Immuno histochemistry - Immuno cytochemistry

ICC is particularly suitable to answer questions related to the localization of signal transduction markers. ICC is routinely performed on brain sections that can best be prepared by means of frozen sectioning. The availability of primary antibodies or radioligands directed against neurotransmitters, cell surface receptors and intracellular receptors, intracellular proteins, peptides etc. these days is nearly unlimited. We have experience with these techniques, with particular interest in receptor systems, G-proteins, kinases and phophatases, neuropeptides, neurotransmitters and neurotransmitter-specific enzymes, cytoskeletal proteins; to mention only a few groups of biomarkers that are being studied.

Immuno histochemistry - Western Blotting

Blotting of proteins or peptides, commonly named 'Western blotting', is the method of choice whenever information is needed on protein identification and protein quantitation.

Protein blotting

Western blotting can be used a biochemical quantitation method and a topographical localization technique. Western blotting can be performed both on freshly prepared brain-slices and on homogenized brain-tissue. After tissue sample homogenization and they can be subjected to a number of separation treatments to select, for example membrane bound proteins or cytoplasmic proteins. After poly acrylamide gel electroforeses and transferal to a membrane antibodies can be used to detect and qunatify a protein/receptor of interest.

Other methods

Our staff has been trained on a number of other molecular techniques, please contact us for other possibilities of detection of your gene / mRNA or protein of interest.